General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0335E2CP2M)
The PathHunter GPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of GPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR1 and EA-tagged β-Arrestin-2. Activation of GPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCR-RAMP2 (AMY2) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0267E2CP0M)
The PathHunter CALCR-RAMP2 (AMY2) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human SDF-1 ASY
The Meso Scale Discovery U-PLEX Human SDF-1alpha Assay Kit is a multiplex immunoassay that measures SDF-1alpha levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and reagents needed to run the assay Results are obtained in as little as 3 5 hours This assay allows for the quantitative measurement of SDF-1alpha levels for life science research applications
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 500. (93-0563R20B)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress Keap1-NRF2 Nuclear Translocation Assay, 2-plate Kit. (93-0821E3CP0M)
The PathHunter eXpress Keap1-NRF2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Keap1-NRF2 pathway. This kit assesses ligand (e.g. methyl CDDO) based pathway activation via detection of resulting NRF2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged NRF2 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of NRF2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR12 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0329E2ACP1M)
The PathHunter GPR12 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR12 and EA-tagged β-Arrestin-2. Activation of GPR12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PDGFRa Functional Assay, 10-Plate Kit. (93-0823E3CP16L)
The PathHunter eXpress PDGFRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PDGFRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 17 Reagent, 100. (93-0563R17A)
AssayComplete Cell Plating 17 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress RXFP1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0127E2CP2L)
The cAMP Hunter RXFP1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-2) based activation of RXFP1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP1, a naturally Gs coupled GPCR. Upon activation of RXFP1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 11 Reagent, 100. (93-0563R11A)
AssayComplete Cell Plating 11 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress ADORA3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0586E2CP2L)
The PathHunter ADORA3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MECA) based activation of ADORA3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADORA3 and EA-tagged β-Arrestin-2. Activation of ADORA3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress NPFFR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0097E2CP2M)
The cAMP Hunter NPFFR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide FF, RFRP-3) based activation of NPFFR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPFFR1, a naturally Gi/Go coupled GPCR. Upon activation of NPFFR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human AXL/UFO ASY
The R-PLEX Human AXL UFO Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that allows for the simultaneous quantitative measurement of AXL in human serum plasma and cell culture supernatants It utilizes Meso Scale Diagnostics electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized calibrator It can test up to 80 samples in duplicate and has a testing time of 3 5 hours
Meso Scale Discovery U-PLEX Mouse IL-17C ASY
The Meso Scale Discovery U-PLEX Mouse IL-17C Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse interleukin 17C (IL-17C) in serum plasma and cell culture supernatants The assay utilizes MSD s proprietary electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay allows for the accurate and reliable measurement of IL-17C which plays an important role in mucosal immunity and inflammation With minimal hands-on time required this kit serves as a useful tool for mouse IL-17C detection in research studies involving immunology and inflammation
Eurofins Discoverx PathHunter eXpress HTR1A CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0696E2CP0L)
The PathHunter HTR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1A and EA-tagged β-Arrestin-2. Activation of HTR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.